D3949-11
DNA PK, Catalytic Subunit (DNA-dependent Protein Kinase)
Size
£971.00
- SKU:
- D3949-11
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human DNA-dependent Protein Kinase purified from HeLa cells.
- Clone:
- 18–2
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See D3949-11X
- Extra Details:
- DNA-dependent Protein Kinase (DNAPKcs) phosphorylates transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks. MW of Antigen: 460kD Epitope: aa1-2713 Applications and Recommended Dilutions: Inhibits (50%) DNA-dependent Phosphorylation Immunofluorescence Immunoprecipitation (Native and denatured) (Use Protein G; Ab at 2ug/mg protein lysate) Western Blotting (Ab 1-2ug/ml for 2hrs at RT) Immunohistochemistry (Formalin/paraffin) (Ab 2-4ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. Positive Control: LS174T cells. Tonsil. Cellular Localization: Nuclear Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice