D3948-01
DNA Primase (p49)
Size
£754.00
- SKU:
- D3948-01
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Purified mouse recombinant DNA primase, p49.
- Format:
- Supplied as a liquid in PBS, pH 7.5,, 0.2% BSA 0.05% sodium azide before the addition of glycerol to 40%.
- Extra Details:
- In the eukaryotic cell, DNA primase initiates DNA replication by the synthesis of small ribonucleotides called primers. The eukaryotic primase is composed of two subunits, p49 (human 420aa; chromosome 12q13) and p58 (human 509aa, chromosome 6p12-p11.1), which purify as a complex tightly bound to DNA polymerase alpha. Primase initiates synthesis with a triphosphate purine moiety at the 5'-end. After synthesis of 7-10 ribonucleotides, the primer-template is translocated intramolecularly to the active site of the DNA polymerase alpha subunit. The p49 subunit of DNA primase contains the catalytic active site. Residue 104-111 are most critical for primer synthesis. Alanine substitution in residues Glu, Asp, and these residues may form part of a conserved carboxylic triad also observed in the active sites of DNA polymerases and reverse transcriptases. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells or other tumor cells Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice