D3940
DNA Polymerase I Klenow Fragment
Size
£422.00
- SKU:
- D3940
- Molecular Weight:
- 62
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- CAS Number:
- 9012-90-2
- Extra Details:
- DNA Polymerase I Large Fragment exhibits the 5’ ---> 3’ polymerase and 3’ ---> 5’ exonuclease activity of DNA Polymerase. Lacks 5’ ---> 3’ exonuclease activity. Applications: • Random primer labeling • Sanger sequencing • cDNA Second Strand Synthesis • 3’ end-labeling, filling • Blunting of DNA ends: fill-in 5'-overhangs or/and removal of 3'-overhangs, see protocol • Oligonucleotide-directed site-specific mutagenesis. Source: E. coli cells with a cloned fragment of the polA gene Storage Buffer: Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 0.1mM EDTA, 1mM DTT, 50% glycerol. Unit Definition: 1u of DNA Polymerase I Klenow Fragment catalyzes the incorporation of 10nmoles of deoxynucleotides into polynucleotide fraction in 30 minutes at 37°C. Reaction Buffer (Supplied): D3940A DNA Polymerase I Klenow Fragment, Reaction Buffer, 10X: Supplied as a liquid in 500mM Tris-HCl, pH 8.0, 50mM magnesium chloride, 10mM DTT Inhibition and Inactivation: • Inhibitors: metal chelators, nucleotide analogs PPi, Proprietray information (at high concentrations). • Inactivated by heating at 75°C for 10 min or by addition of EDTA. Quality Control: Endodeoxyribonuclease Assay: No detectable degradation was observed after incubation of supercoiled plasmid DNA with DNA Polymerase I Klenow Fragment. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice