D3935-35A
DNA Polymerase, gamma
Size
£927.00
- SKU:
- D3935-35A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human recombinant DNA Pol gamma aa26-276
- Clone:
- 3H16
- Format:
- Supplied as a liquid in 10 mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.
- Extra Details:
- Mitochondria produce cellular ATP through oxidative phosphorylation. Mitochondrial DNA encodes several proteins necessary for oxidative phosphorylation, whereas many other genes are nuclear. DNA Pol gamma (POLG), the only DNA polymerase present in mitochondria, has been implicated in base excision repair process. The catalytic subunit of POLG catalyzes the release of dRP (5' terminal deoxyribose phosphate) from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. POLG deficiency has been associated with mtDNA depletion and Alper's syndrome. Low selectivity of mtDNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml. The antibody recognizes >140kD DNA Pol g from HeLa and other cells and tissues. ELISA: 1:5000-25,000 using 50-100ng control antigen/well. Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice