D3935-28X

DNA Polymerase, epsilon (e)

Size

£927.00

SKU:: D3935-28X
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified Human recombinant DNA Pol epsilon aa1-176
Clone:: DNPE12
Format:: Supplied as a liquid in 10 mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: Reactive oxygen species (H2O2·, OH·, O2, HOCL) generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents can induce a wide variety of lesions in DNA ranging from oxidized bases (8-oxoguanine and thymine glycol), base loss and DNA strand breaks with modified ends such as 3'-phosphate and 3'-phopshoglycolate. Unrepaired damaged/modified DNA has been implicated in aging and cancer. DNA Pol epsilon is expressed in proliferating cells. It is implicated in repair of UV-damaged DNA. DNA Pol epsilon is a protein of >200kD protein complex. The 140-KDa fragment, DNA Pol e* is a truncated form of DNA Pol e that is missing the C-terminal fragment. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.