D3935-21
DNA Polymerase, delta, catalytic subunit (Pol D) (Not for Export EU)
Size
£704.00
- SKU:
- D3935-21
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 21-aa peptide sequence mapping near the N-terminus of human Pol D (1) was synthesized, coupled to KLH
- Format:
- Supplied as a liquid, neat serum, 40% glycerol.
- Extra Details:
- Reactive oxygen species (H2O2·, OH·, O2, HOCL) generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents can induce a wide variety of lesions in DNA ranging from oxidized bases (8-oxoguanine and thymine glycol), base loss and DNA strand breaks with modified ends such as 3'-phosphate and 3'-phopshoglycolate. Unrepaired damaged/modified DNA has been implicated in aging and cancer. In eukaryotes, there are five DNA polymerases (alpha, beta, gamma, delta, and epsilon), which are responsible for different reactions in DNA synthesis. DNA polymerase d (Pol d) is the key polymerase that is in involved in replication of chromosomal DNA. It has two enzymatic activities: DNA synthesis (polymerase) and an exolytic activity that degrades ssDNA in the 3'-5' direction. DNA Pol d is tetrameric complex of 4 subunits: a large catalytic subunit Pol3 ~p125kD and three smaller subunits of p50, p68 and p12kD. Pol D requires accessory proteins, PCNA and RFC or activator 1 for enzymatic activity. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-50,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Control Peptide: O3935-18 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice