D3935-18

DNA Polymerase, delta, catalytic subunit, Human, Control Peptide (Pol D)

Size

£384.00

SKU:: D3935-18
Purity:: Highly purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.2
Extra Details:: Control peptide for D3938-21 (serum) A 21-aa peptide sequence (designated DNPD11-P, control or blocking peptide) mapping near the N-terminus of human Pol D (1) was synthesized, coupled to KLH, and polyclonal antibodies generated in rabbits. Reactive oxygen species (H2O2·, OH·, O2, HOCL) generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents can induce a wide variety of lesions in DNA ranging from oxidized bases (8-oxoguanine and thymine glycol), base loss and DNA strand breaks with modified ends such as 3'-phosphate and 3'-phopshoglycolate. Unrepaired damaged/modified DNA has been implicated in aging and cancer. In eukaryotes, there are five DNA polymerases (alpha, beta, gamma, delta, and epsilon), which are responsible for different reactions in DNA synthesis. DNA polymerase d (Pol d) is the key polymerase that is in involved in replication of chromosomal DNA. It has two enzymatic activities: DNA synthesis (polymerase) and an exolytic activity that degrades ssDNA in the 3'-5' direction. DNA Pol d is tetrameric complex of 4 subunits: a large catalytic subunit Pol3 ~p125kD and three smaller subunits of p50, p68 and p12kD. Pol D requires accessory proteins, PCNA and RFC or activator 1 for enzymatic activity. Applications: Suitable for use ELISA. Other applications not tested. Recommended Dilution: ELISA: 50-100ng control peptide/well Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffe

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