D3935-12A
DNA Polymerase, beta
Size
£927.00
- SKU:
- D3935-12A
- Purity:
- Purified by protein A/G affinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified Rat DNA Pol beta (~39kD)
- Clone:
- 3H14
- Format:
- Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
- Extra Details:
- Reactive oxygen species (H2O2·, OH·, O2, HOCL) generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents can induce a wide variety of lesions in DNA ranging from oxidized bases (8-oxoguanine and thymine glycol), base loss and DNA strand breaks with modified ends such as 3'-phosphate and 3'-phopshoglycolate. Unrepaired damaged/modified DNA has been implicated in aging and cancer. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed. DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MMNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. DNA polymerase beta comprises an amino-terminal 8-kD domain and a carboxy-terminal 31kD domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml. The antibody has detected ~39kD band from HeLa and other cells and tissues. ELISA: 1:10,000-50,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice