D3931-70U
Dnmt3a (DNA (Cytosine-5)-methyltransferase 3A, Dnmt3a, DNA Methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA)
Size
£863.00
- SKU:
- D3931-70U
- Application:
- WB
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to aa353-486 from human DNMT3A expressed in E.coli.
- Clone:
- 12K80
- Format:
- Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
- Extra Details:
- DNMT3A is a 130kD member of the C5-methlytransferase family of molecules. It is expressed both during development and in virtually all adult tissues except for small intestine. DNMT3A is a nuclear protein that is found associated with heterochromatin. It possesses DNA methyltransferase activity, and acts in a complex with DNMT3L in embryonic stem cells, forming a (3A)2:(3L)2 heterotetramer. It also interacts with SETDB1 to form a transcriptional repressor. Human DNMT3A is 912aa in length. It contains one ProTrpTrpPro domain aa292-350 and one ADD-type zinc finger region aa494-586. There is one 100kD splice variant termed DNMT3a2 that contains an alternative start site at Met224, and preferentially interacts with euchromatin. Other potential splice variants show a 16aa substitution for aa151-912, and a 24aa substitution for aa1-213. Over aa353-486, human DNMT3A shares 96% aa identity with mouse DNMT3A. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice