D3931-63B

DNA Methyltransferase 1 (DNMT, MCMT, M.Hsa1, DNA (Cytosine-5-)-methyltransferase 1, DNA MTase Mmu1, M.HsaI)

Size

£642.00

SKU:: D3931-63B
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-RFESPPKTQPTEDN from an internal region of human DNMT1. Species sequence homology: mouse, rat, canine, bovine and porcine.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:32,000. Western Blot: 0.5-1.5ug/ml. A band at ~170kD band observed in lysates of cell line Jurkat. Immunohistochemistry: 3-6ug/ml. In paraffin embedded Human Kidney shows nuclear staining in PCT. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.