D3925-01A

DNA Ligase 1

Size

£927.00

SKU:: D3925-01A
Purity:: Purified by Protein A/G column chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified bovine DNA ligase-I protein
Clone:: 3H13
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Extra Details:: Eukaryotic DNA ligases are ATP-dependent enzymes that catalyze the joining of single and double stranded DNA breaks, which is an essential step in DNA repair, replication and recombination. There are at least 4 DNA ligases, termed DNA ligase I-IV, have been cloned and characterized. DNA ligase-I is functionally homologous to the DNA ligase encoded by the S. cervisiae CDC9 gene. The joining of Okazaki fragments during lagging strand DNA replication is due to DNA ligase-I. DNA ligase-I (919 aa, chromosome 19q13.2-q13.3; mouse 916 aa, rat 919 aa) is a nuclear protein. Its deficiency causes immunodeficiency and cellular hypersensitivity of DNA-damaging agents. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.