D3778-01
Disulfide Oxidoreductase, Recombinant, E. coli (dsbA)
Size
£819.00
- SKU:
- D3778-01
- Molecular Weight:
- 21.1
- Purity:
- ≥95% by SDS PAGE
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 20mM Tris•HCl, pH 7.5, 2mM EDTA
- Extra Details:
- Recombinant Disulfide Oxidoreductase (rDsbA), produced from E. coli is a periplasmic protein and thioredoxin superfamily member which introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids). Recombinant protein corresponding to DsbA (residues 20-208) was expressed in E. coli and purified by using conventional chromatography techniques. Molecular Weight: ~21.1kD (189 amino acids) Amino Acid Sequence: MAQYEDGKQY TTLEKPVAGA PQVLEFFSFF CPHCYQFEEV LHISDNVKKK LPEGVKMTKY HVNFMGGDLG KDLTQAWAVA MALGVEDKVT VPLFEGVQKT QTIRSASDIR DVFINAGIKG EEYDAAWNSF VVKSLVAQQE KAAADVQLRG VPAMFVNGKY QLNPQGMDTS NMDVFVQQYA DTVKYLSEKK Enzyme Activity: Not Determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice