D3441-87D

DGKI (Diacylglycerol Kinase iota, DAG Kinase iota, Diglyceride Kinase iota, DGK-iota)

Size

£825.00

SKU:: D3441-87D
Application:: WB
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Ser925-Val1065 (predicted) from human DGK-i, expressed in E. coli.
Clone:: 11n60
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Diacylglycerol kinase iota (DGK-i) is an ~110kD kinase that phosphorylates the lipid diacylglycerol. DGK-i also inhibits RasGRP3 and limits Rap1 activation. It contains two zinc finger regions (aa178-232-aa251-310, a catalytic domain aa372-507, and two ankyrin repeats aa958-990-aa997-1026. Over aa925-1065, human DGK-i shares 94-96aa sequence identity with mouse and rat DGK-i, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using lysates of SH-SY5Y human neuroblastoma cell line or mouse brain tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.