D3441-82B
DGKB (Diacylglycerol Kinase beta, Diglyceride Kinase beta, DGK-beta, DAG Kinase beta, 90kD Diacylglycerol Kinase, DAGK2, KIAA0718)
Size
£800.00
- SKU:
- D3441-82B
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Ser143 from human DGKB expressed in E. coli.
- Clone:
- 11n59
- Format:
- Supplied as a lyophilized powder from PBS, pH .4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Diacylglycerol kinase beta (DGK-b) is an ~90kD kinase that phosphorylates the lipid diacylglycerol. It is expressed in the brain and colocalizes with D1 and D2 dopamine receptors. DGK-b promotes the formation and maturation of dendritic spines. It contains two EF-hand motifs (aa149-184, 194-229), two zinc finger regions (aa244-294, aa309-358), and a catalytic domain (aa434-568). Alternate splicing generates an additional isoform with a 31aa C-terminal deletion. Over aa 1-143, human DGK-b shares 97aa sequence identity with mouse and rat DGK-b. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml U87s MG human glioblastoma/astrocytoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice