D3375-03

Diamine Oxidase (DAO, Amiloride-sensitive amine oxidase (copper-containing), EC=1.4.3.22, Amiloride-binding protein, Histaminase, Kidney amine oxidase, ABP, ABP1, AOC1, DAO1) (Not for Export EU)

Size

£460.00

SKU:: D3375-03
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding human Diamine oxidase (DAO)
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 20ul or 100ul sterile ddH2O.
Extra Details:: Diamine oxidase (DAO) is a flavoprotein/enzyme which catalyzes the aerobic oxidation of amines to the corresponding aldehyde and ammonia. Diamine oxidase is synthesized primarily in the gastrointestinal mucosal cells. Serum levels of DAO have been used as an indicator of the integrity and/or functional mass of the intestinal mucosa. The enzyme is also produced by the placenta and is elevated in newborn serum. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:4000 Western Blot: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.