D3224-32A
DFF40 (CAD I18, DNA Fragmentation Factor, Caspase Activated Deoxyribonuclease, CPAN)
Size
£537.00
- SKU:
- D3224-32A
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Peptide corresponding to aa205-222 of human DFF40. Species Sequence Homology: mouse
- Clone:
- 36A349
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
- Extra Details:
- Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribo-nuclease)(1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40(3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively(5). Upon cleavage of DFF45/ICAD by activated caspases, DFF40/CAD is released and activated and eventually causes degradation of DNA in the nuclei1(4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:2000, HeLa cell lysate can be used as positive control and a 40kD band is detected. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice