D3221-94T1
Developmental Pluripotency-Associated Protein 5 (DPPA5, ECAT2, Esg-1) (Biotin)
Size
£863.00
- SKU:
- D3221-94T1
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- E. coli-derived rmDPPA5
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Extra Details:
- DPPA5, also known as Esg-1 and ECAT2, is a 14kD RNA-binding protein expressed in early embryos, primordial germ cells, and embryonic stem cells and is used as a marker of the pluripotent state. Mouse DPPA5 is 118 amino acids in length and shows 63% amino acid identity with human and 81% amino acid identity with rat DPPA5 proteins. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 25% cross-reactivity with rhDPPA5 and less than 10% cross-reactivity with rmDPPA4. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice