Developmental Pluripotency-Associated Protein 4 (DPPA4)

Size

£537.00

SKU:: D3221-94R4
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Portion of aa1-50 of human DPPA4. Species sequence homology: monkey.
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Extra Details:: Developmental pluripotency associated 4 (DPPA4) protein is abundantly found in embryonic stem cells and in early embryos. DPPA4 protein is localized in the embryonic stem cell nucleus associated with transcriptionally active chromatin. The Dppa4 gene overexpression does not support embryonic stem cell self-renewal but partially inhibits embryonic stem cell differentiation. Suppression of DPPA4 expression by short hairpin RNA induced embryonic stem cell into a primitive ectoderm lineage. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2-4ug/ml Immunohistochemistry (formalin fixed paraffin embedded): -5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.