D3221-83A
Desmoglein 2 (DSG2, ARVC10, ARVD10, CDHF5, HDGC)
Size
£856.00
- SKU:
- D3221-83A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain).
- Clone:
- 5F76
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
- Extra Details:
- Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein1-3 and desmocollin1-3. Desmosomal cadherins showed differentiation -specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Domain specific desmosomal and adherens junction-associated antibodies have been widely used in recent studies. In one study, domain specific antibodies were used to determine the cellular localization of the skin of patients with Hailey-Hailey disease and Darier’s disease. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:10 with PBS, pH 7.4; Incubation: 1 hr at RT. Note: For better resolution on frozen sections preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, is recommended. Immunohistochemistry (Paraffin): After microwave treatment, dilute 1:10 with PBS, pH 7.4; Incubation: Overnight at 4°C. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice