D3221-15D

Desmin (DES, CMD1I, CSM1, CSM2, Intermediate Filament Protein)

Size

£960.00

SKU:: D3221-15D
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified porcine desmin
Clone:: DE-R-11
Format:: Supplied as liquid tissue culture supernatant in 15mM sodium azide
Extra Details:: Desmin is a muscle protein found in skeletal, cardiac and smooth muscle cells. This antibody will help determine the presence of desmin and identify cells of myogenic origin in uncharacterized tissues. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:250 Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): 1:50-1:100 for 1 hour at RT. Trypsin digestion is recommended. The recommended fixative is 10% neutral-buffered formalin for paraffin-embedded tissue sections. Heat Induced Epitope Retreival (HIER) was performed in Epitope Retrieval Solution pH 9. Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: Muscle Immunohistochemistry: Intestine wall (cytoplasmic staining pattern) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.