D3221-14
Desmin, Recombinant, Human (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
Size
£863.00
- SKU:
- D3221-14
- Molecular Weight:
- 53.5396
- Purity:
- ≥95% (determined by SDS gel electrophoresis).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder. Reconstitute with 70ul sterile water. Final Solution contains: 30mM Tris-HCI, pH 8, 9.5M urea, 2mM DTT, 2mM EDTA, 10mM methylammonium chloride.
- Extra Details:
- Desmin is the main intermediate filament in mature skeletal, cardiac and smooth-muscle cells. Can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas and carcinomas. Molecular Weight: Calculated from sequence: 53.5396kD Observed (SDS Gel Electrophoresis): 55kD Isoelectric Point: pI 5.16 (calculated from sequence). Applications: Suitable for use as protein standard in 1D and 2D SDS gel electrophoresis, in Immunoassays and in immunization. Other applications not tested. Reconstitution to Filaments: Protofilaments and filament complexes are obtained by dialyzing the reconstituted polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM sodium chloride, 2mM DTT, 10mM Tris-HCl, pH 7.4) For Use in Immunization: The solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). Storage and Stability Store lyophilized product at -20°C. Store reconstituted product in aliquots at -20°C to avoid repeated freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice