D3206-30

DEP1, protein tyrosine phosphatase, catalytic domain, Recombinant, Human, aa1024-1306 (Density-enhanced Phosphatase-1, PTP-eta, CD148)

Size

£918.00

SKU:: D3206-30
Purity:: >95% by SDS-PAGE.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25 mM Tris-HCl, pH 8.0, 75 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 2mM EDTA, 3mM DTT.
Extra Details:: Protein tyrosine phosphatase DEP1 (density-enhanced phosphatase-1), (GenBank accession no. NM_U10886), a.a. 1024-1306 (catalytic domain) with N-terminal GST-tag, MW=58.9 kDa, expressed in an E. coli expression system. Specific Activity: 114 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50mM pNPP. Application: useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.