D3200-07
Deoxyribonuclease I (DNase I) (Biotin)
Size
£889.00
- SKU:
- D3200-07
- Purity:
- Purified by delipidation, salt fractionation and ion exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Bovine pancreatic Deoxyribonuclease I
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 100ul sterile ddH2O. Labeled with Biotin.
- Extra Details:
- Deoxyribonuclease I (DNase I) is a Ca2+ and Mg2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: ELISA: 1:5000-1:20,000. Assayed against 1ug of Deoxyribonuclease I a standard capture ELISA using Streptavidin (HRP) and ABTS as a substrate for 30 minutes at RT. Western Blot: 1:500-1:5000 Immunoprecipitation: 1:100 Optimal dilutions to be detemined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice