D3200-06

Deoxyribonuclease I (DNase I)

Size

£914.00

SKU:: D3200-06
Purity:: Purified by Ion Exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Bovine pancreatic Deoxyribonuclease I
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Reconstitute with 100ul sterile dH2O.
Extra Details:: Deoxyribonuclease I (DNase I) is a Ca 2+ and Mg 2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo-and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Conjugation. Other applications have not been tested. Recommended Dilutions: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:5000 Immunoprecipitation: 1:100 Optimal dilutions to be detemined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.