D3200-05A

Deoxyribonuclease I (DNase I)

Size

£767.00

SKU:: D3200-05A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Purified bovine pancreatic DNase I (EC 3.1.21.1).
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Extra Details:: Deoxyribonuclease I (DNase I) is a Ca 2+ and Mg 2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000 Western Blot: 1:500-1:2000; D3200-05B can be used as a positive control. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.