D2727-75C-BIOTIN
DLL1 (Delta-like Protein 1, Drosophila Delta Homolog 1, Delta1, H-Delta-1, UNQ146/PRO172) (Biotin)
Size
£837.00
- SKU:
- D2727-75C-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Hamster
- Immunogen:
- DLL1 expressing CHO cells.
- Clone:
- HMD1-5
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, which is vital for cellcell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic Tcell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Deltalike protein1(DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAMfamily metalloproteases and the gammasecretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSLNICDMastermind complex cascade (canonipathway),NFkappaBNICD and CSLNICDDeltex complex signaling cascades (noncanonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the noncanonical pathway promotes differentiation.DLL1 is widely expressed, and acts as a mediator of cell fate decisions during hematopoiesis, and may play a role in celltocell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signaling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB). Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections. Suitability for use with paraffin sections has not been determined. Optimal dilutions to be determined by the researcher. Hybridoma: P3U1 myeloma cells with spleen cells from Armenian hamsters. Positive Control Tissue: Mouse spleen Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice