Defender of Apoptotic Cell Death 1 (DAD1)

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£678.00

SKU:: D1877-15B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a 14aa sequence within the C-Terminal region of human DAD1. Species Sequence Homology: rat, porcine, bovine and chicken. Epitope: last 50aa.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Extra Details:: Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains. Applications: Suitable for use in Western Blot, ELISA, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml, detects a band of ~22kD in HepG2 cell lysates. Immunocytochemistry: 10ug/ml. Immunofluorescence: 10ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice