D1876-48Q

Dectin-1 (C-type Lectin Domain Family 7 Member A, Dendritic Cell-associated C-type Lectin 1, DC-associated C-type Lectin 1, Beta-glucan Receptor, CANDF4, C-type Lectin Superfamily Member 12, CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082)

Size

£550.00

SKU:: D1876-48Q
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: NIH3T3 cells expressing full length Beta-glucan receptor.
Clone:: GE2
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Dectin-1, also known as beta-glucan receptor (ßGR), is a C-type lectin domain family member. It is a major pathogen pattern-recognition receptor that binds beta-glucans, glucose polymers that form cell-wall components or exopolymers of yeasts, fungi and some bacteria. Beta-glucans have also been used experimentally and therapeutically as immunomodulators that enhance resistance to bacterial, yeast, viral and protozoan infections, as well as tumor formation. Dectin-1 also acts as a co-stimulatory molecule on T-cells to induce their proliferation and is necessary for the TLR2-mediated inflammatory response. It is highly expressed on peripheral blood leukocytes and dendritic cells. Human Dectin-1 has two major (βGR-A and βGR-B) and several minor isoforms. βGR-A and βGR-B differ by the presence and absence, respectively, of a stalk region. Clone 9i100 recognizes both major isoforms, and could be used to inhibit receptor function. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Sp2 myeloma cells with spleen cells from C57BL/6 mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice