D1876-48P2
Dectin-1 (C-type Lectin Domain Family 7 Member A, Dendritic Cell-associated C-type Lectin 1, DC-associated C-type Lectin 1, Beta-glucan Receptor, beta-GR, C-type Lectin Superfamily Member 12, CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082)
Size
£754.00
- SKU:
- D1876-48P2
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
- Clone:
- 10B2689 (2A11)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- As a major leukocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): The epitope recognized is sensitive to H2O2 treatment, so quenching with 0.3% H2O2 should be performed after incubation with the primary antibody. The epitope is also sensitive to formaldehyde fixation and tissue processing. Acetone fixation of frozen sections is recommended. Optimal dilutions to be determined by the researcher. Hybridoma: Rat Y3 myeloma cells with spleen cells from Fischer rats Recommended Secondary Antibodies: I1904-47Y: IgG, Fc (HRP) Pab Gt xRt I1904-47T: IgG, H&L (HRP) Pab Gt xRt I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRt I1904-47H: IgG, H&L (HRP) Pab Gt xRt I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab Gt xRt Recommended Negative Control: I1904-82V4: IgG2b, Rat Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice