D1876-48A
Dectin-1, Low Endotoxin (C-type lectin domain family 7 member A, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Beta-glucan receptor, C-type lectin superfamily member 12, CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082)
Size
£971.00
- SKU:
- D1876-48A
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- NIH3T3 cells expressing full length Betaglucan receptor.
- Clone:
- GE2
- Format:
- Supplied as a liquid in PBS, pH 7.4. No preservative added.
- Extra Details:
- Dectin-1, also known as beta-glucan receptor (bGR), is a C-type lectin domain family member. It is a major pathogen pattern-recognition receptor that binds beta-glucans, glucose polymers that form cell-wall components or exopolymers of yeasts, fungi and some bacteria. Beta-glucans have also been used experimentally and therapeutically as immunomodulators that enhance resistance to bacterial, yeast, viral and protozoan infections, as well as tumor formation. Dectin-1 also acts as a co-stimulatory molecule on T-cells to induce their proliferation and is necessary for the TLR2-mediated inflammatory response. It is highly expressed on peripheral blood leukocytes and dendritic cells. Human Dectin-1 has two major (bGR-A and bGR-B) and several minor isoforms. bGR-A and bGR-B differs by the presence and absence, respectively, of a stalk region. D1876-48A recognizes both of these isoforms, and it could be used to inhibit receptor function. Applications: Suitable for use in Flow Cytometry and Funcional Assays. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat -1:25; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Endotoxin: ≤0.1EU/ug Hybridoma: Spleen cells from immunized C57BL/6 mice were fused with cells of the murine SP2 myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice