DDX6 (DDX-6, DEAD-box protein 6, DEAD (Asp-Glu-Ala-Asp))

Size

£642.00

SKU:: D1661-04
Purity:: Purified by peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-AEYHSEPVEDEKP, from the C Terminus of human DDX6 (NP_004388.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:16,000 Western Blot: 0.1-0.3ug/ml ~ 50kD band observed in lysates of cell line Jurkat (calculated MW of 53.2kD according to NP_NP_004388.1). In transfected HEK293 transiently expressing Human DDX6 a band of approx. 55kD is observed. This band is not observed in the non-transfected HEK293. The endogenous 53kD protein is observed in both. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.