DDB1 (DDB1, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, damage-specific DNA binding protein 1, 127kDa, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD))

Size

£642.00

SKU:: D1575-01B
Purity:: Purified by peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-DLIKVVEELTRIH, from the C Terminus of human DDB1.. Species sequence homology: rat, canine, bovine.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:32,000 Western blot: 0.5-1.5ug/ml ~130kDa band seen in HeLa, Jurkat and NSO (mouse) cell lysates. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice