D1550-03G

DC-SIGN (Dendritic Cells, CD209, Dendritic Cell-Specific ICAM-3, SIGNR1, SIGN-R1, Dendritic Cell-specific ICAM-3-grabbing Non-integrin)

Size

£869.00

SKU:: D1550-03G
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: NIH/3T3 transfected with recombinant, human DC-SIGN. Cellular Localization: Cell Membrane and Secreted.
Clone:: 8J1270
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 5% trehalose. Reconstitute in 1ml sterile PBS.
Extra Details:: Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Adhesion Blockade and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry (Immunophenotyping): 10ul of 25-50ug/ml labels up to 10e6 cells Adhesion Blockade: 5ug/ml (5x10e6 cells/well). Immunohistochemistry: 8-25ug/ml using Immersion fixed paraffin-embedded sections of human lymph node Western Blot: 1ug/ml, using Recombinant Human DC-SIGN Fc Chimera Optimal dilutions to be determined by the researcher. Positive Control: Transfected NIH/3T3 cells and monocyte derived dendritic cells. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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