D1499
DcR1 (TRAIL-R3, TRID, LIT, TRAIL Decoy Receptor1)
Size
£655.00
- SKU:
- D1499
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to amino acids in a extracellular domain of human DcR1 precursor. Epitope: aa140-190
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT (2-7) and DcR2/TRAIL-R4/ TRUNDD (8-10). DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues. But not in most cancer cell lines (2,3) Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis (2,3). Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Immunocytochemistry: 10ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysate from HeLa cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice