D1499-01
DcR1 (Decoy Receptors for TRAIL, ED2, TRAIL-R3, TRID, LIT)
Size
£655.00
- SKU:
- D1499-01
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to amino acids in the extracellular domain of human DcR1 precursor. Epitope: aa90-140
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL. Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain, but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts are expressed in many normal human tissues, but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis. Applications: Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, detects a ~65kD band Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice