D1100-01A

Datura stramonium (DSL)

Size

£614.00

SKU:: D1100-01A
Purity:: Homogeneous by SDS-PAGE
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. Reconstitute with 10mM HEPES buffered saline, pH 8.5, 0.1mM CaCl2. Do not vortex.
Extra Details:: The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL also binds well to Nacetyllactosamine and oligomers containing repeating -acetyllactosamine sequences. Abranched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Sugar Specificity: [GlcNAc]1-3, N-Acetylglucosamine Inhibiting/Eluting Sugar: Chitin Hydrolysate Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile 10mM HEPES buffered saline, pH 8.5, 0.1mM CaCl2. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.