DARC (CT) (Duffy Antigen/Chemokine Receptor, Duffy Antigen/Chemokine Receptor, Fy Glycoprotein D, GpFy, Cell Adhesion Molecule 3, CADM3, Plasmodium Vivax Receptor,CCBP1, CD234, CD234 antigen, Dfy, GpFy, Plasmodium vivax receptor) (HRP)

Size

£869.00

SKU:: D1065-50H-HRP
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a 16aa sequence near the carboxy terminus of human DARC. Epitope: within the last 50aa.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: CD234 is a non-specific receptor for a number of cytokines including IL-8, GRO, RANTES, MCP-1 and TARC. CD234 also functions as the receptor for the malaria parasites Plasmodium vivax and P. knowlesi. CD234 is also responsible for the Duffy blood group system where a substitution at position 42 of either Gly-42 or Asp-42 results in Fy(A) or Fy(B). CD234 is present in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along post capillary venules throughout the body, except in the adult liver. Erythroid cells and post capillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium. It has been demonstrated that CD234 exists in multiple isoforms. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not tested. Recommended Dilution: Immunohistochemistry: Paraffin. Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control Tissue: Mouse brain Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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