D1049-05
DAP5 (Death Associated Protein 5, EIF4g2, Eukaryotic Elongation Factor 4 gamma 2)
Size
£581.00
- SKU:
- D1049-05
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding amino acid 805 of human DAP5.
- Format:
- Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Extra Details:
- Death associated protein 5 (DAP5) is a 97kD protein with high amino acid sequence homology to eukaryotic translation initiation factor 4G (eIF4G) (1,2). Compared with eIF4G, DAP5 lacks the N-terminal region necessary for cap-dependent translation and has a unique C-terminal part functioning as a regulator for interferongamma induced cell death (2,3). During induced apoptosis, DAP5 is cleaved at Asp790. The C-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis (4). Applications: Suitable for use in ELISA and Western Blotting. Other applications not tested. Recommended Dilution: Western Blotting: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice