DAP3 (Death Associated Protein 3)

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£642.00

SKU:: D1049-03F
Purity:: Purified by peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to NPSLLERHCAYL, from the C Terminus of human DAP3 (NP_387506.1; NP_004623.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:16,000 Western Blot: 1-3ug/ml ~40kD band observed in lysates of cell line HeLa (calculated MW of 45.6kD according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48kD is observed. This band is not observed in the non-transfected HEK293. Immunohistochemistry: 2.5ug/ml, paraffin embedded Human Tonsil and Brain (Cortex). Immunocytochemistry: 3-10ug/ml, mitochondrial staining of HeLa after in situ denaturation of the target protein using 3-4M guanidinium thiocyanate The signal goes down upon knockdown of the DAP3 expression. Immunofluorescence: 5ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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