D0101-01
D4-GDI (Cleavage Specific) (D4 GDP-Dissociation Inhibitor)
Size
£633.00
- SKU:
- D0101-01
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI.
- Clone:
- 4H74 (97A1015)
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Extra Details:
- D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kD and 5kD size fragment. The 23kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms. Applications: Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-1ug/ml Immunoprecipitation: 1-2ug/ml Flow Cytometry: 0.5-1ug/1x10e6 cells Immunohistochemistry (Paraffin): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice