Custom Protein Expression, E. coli

Size

POA

SKU:: CS090
Supplier:: United States Biological
Extra Details:: E. coli Expression: United States Biological offers a recombinant protein expression service in E. coli. Our high-level recombinant protein expression system includes scale up as requested, tag removal as requested, fast turn-around time, and competitive pricing. Phase I: Cloning of your interest gene into bacterial expression vector with His-tag or other selected tag (such as GST or thioredoxin). *Amplification of gene of interest out of a customer-supplied vector and subcloning of it into an expression vector. *Verification of the authenticity of the subcloned gene by restriction enzyme digestion and sequencing. *Transformation of recombinant construct into high efficiency expression bacterial strain. *Mini-induction to over-express the target protein. *Test for the expression of the recombinant protein by SDS-PAGE or/and western blot. Estimated time: 2~4 weeks Phase II Large-scale Culture and Purification: Up to 1Liter of bacterial culture will be induced with IPTG and harvested for protein purification. Protein purification using Ni-NAT (for His-tagged proteins) or glutathione (for GST-tagged proteins) beads at either native or denatured conditions. Solubility not guaranteed Estimated time: 2 weeks