Custom Protein Expression, Baculovirus

Size

POA

SKU:: CS090-05
Supplier:: United States Biological
Extra Details:: Baculovirus Expression: United States Biological offers a baculovirus recombinant protein expression service. Our high-level baculovirus expression system includes fast turn-around time, online progress tracking, and competitive pricing. Breakdown of Standard Expression: Phase I: * Amplification/isolation of the gene of interest out of a customer-supplied vector and subcloning of it into a transfer vector with His-tag. * Sequencing confirmation of insert. * Transformation of E. coli with baculoviral DNA and gene of interest. * Culture of E. coli and purification using mini-prep to produce bacmid (recombinant viral DNA). * Transfection of sf9 insect cells with bacmid. * Plaque purification and viral titer assay. * Small-scale culture to test expression level. Estimated time: 4-6 weeks Phase II: * Virus stock preparation * Large-scale culture and infection of insect cells for protein expression. * Purification from 1 liter of infected insect cell using affinity column of Ni-NTA or other column. Estimated time: 2 weeks