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C9111

Cytosine, 5-Methyl

Size

£364.00

SKU:
C9111
Purity:
Purified by Protein G affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Sheep
Immunogen:
5-Methyl Cytosine (KLH).
Format:
Supplied as a liquid in PBS. No preservative added.
Extra Details:
Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 25-100ug/ml Immunohistochemistry (Frozen and paraffin sections): 10ug/ml. Fixation in cold methanol for 30 minutes followed by immersion in 0.007M NaOH for 10-15 seconds allows staining of both nuclear, cytoplasmic and membrane components as well as preservation of morphological detail. Immunocytochemistry: 10ug/ml Immunofluorescence: 10ug/ml Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice