C9099-01
Cytomegalovirus, Late Antigen (CMV)
Size
£672.00
- SKU:
- C9099-01
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Sucrose gradient purified CMV AD169 (ATCC). Epitope: late antigen.
- Clone:
- 1G5.2
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- With CMV the antigens expressed at different times are listed as: IImmediate Early (alpha gene expression): those antigens expressed at 3-12hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60-80kD. Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis. Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein is 55kD. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Not suitable for Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:400-1:800 on acetone fixed cells. Immunohistochemistry: Works on paraffin embedded tissue sections. Dilute with buffer pH 7.4-7.6 to desired working volume. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice