C9099-01-ML650
Cytomegalovirus, Late Antigen (CMV) (MaxLight 650)
Size
£940.00
- SKU:
- C9099-01-ML650
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Sucrose gradient purified CMV AD169 (ATCC). Epitope: late antigen.
- Clone:
- 1G5.2
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. With CMV the antigens expressed at different times are listed as: IImmediate Early (alpha gene expression): those antigens expressed at 3-12hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60-80kD. Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis. Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein is 55kD. Aplications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Not suitable for Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: acetone fixed cells. Immunohistochemistry: Works on paraffin embedded tissue sections. Dilute with buffer pH 7.4-7.6 to desired working volume. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice