C9099-01-BIOTIN
Cytomegalovirus, Late Antigen (CMV) (Biotin)
Size
£940.00
- SKU:
- C9099-01-BIOTIN
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Sucrose gradient purified CMV AD169 (ATCC). Epitope: late antigen.
- Clone:
- 1G5.2
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- With CMV the antigens expressed at different times are listed as: IImmediate Early (alpha gene expression): those antigens expressed at 3-12hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60-80kD. Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis. Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein is 55kD. Aplications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Not suitable for Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: acetone fixed cells. Immunohistochemistry: Works on paraffin embedded tissue sections. Dilute with buffer pH 7.4-7.6 to desired working volume. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice