C9097-44B
Cytokeratin 8,18
Size
£704.00
- SKU:
- C9097-44B
- Application:
- IHC-Fr
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human keratin 8 & 18. Expected Staining Pattern: Cytoplasmic and membrane
- Clone:
- 3G126
- Format:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
- Extra Details:
- Cytokeratins are a family of water-insoluble intermediate filaments, composed of polypeptide chains found in the cytoskeleton of epithelial cells. Twenty different forms of human cytokeratins exist (Moll numbers 1–20) in both normal and malignant epithelial cell lines. (1) Specific combinations of cytokeratin peptides are associated with certain epithelial cells, and are therefore useful in the characterization of poorly differentiated carcinoma. (2–4) Cytokeratins may also be clinically significant indicators in distinguishing carcinoma from malignant tumors of non-epithelial origin, such as lymphoma, sarcoma, and melanoma. Cytokeratin 8 and 18 staining has also been observed in renal cell carcinomas, hepatocellular carcinomas, cholangiocarcinomas, germ cell tumors and some neuroendocrine carcinomas, epithelioid sarcomas and non-epithelial tumors. (7) Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Stains cytokeratins 8 and 18 in frozen tissue or formalin-fixed, paraffin-embedded (FFPE) tissue sections. Optimal dilutions to be determined by the researcher. Epitope retrieval: Required (Citrate buffer, pH 6.0). Enzyme digestion: Required. Control Tissue: Normal colon Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice