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C9097-43A

Cytokeratin 9

Size

£793.00

SKU:
C9097-43A
Purity:
Hybridoma culture supernatant.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Synthetic peptides (1) aa450-477 and (2) N-terminal aa4-28] of human cytokeratin 9 (MW 62,129).
Clone:
5F236, 5F237 (Ks9.70, Ks9.216)
Format:
Supplied as a liquid with 0.09% sodium azide.
Extra Details:
CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. Applications: Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment. Optimal dilutions to be determined by the researcher. Recommended Dilution: Western Blot: 1:25-1:300 (chemiluminescence, AP/ECL). Immunofluorescence Microscopy (Frozen): 1:10 Immunohistochemistry: 1:20 (PAP/ABC), microwave treatment and in some cases protease digestion is recommended Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice