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C9097-43

Cytokeratin 9 (Not for Export EU)

Size

£877.00

SKU:
C9097-43
Purity:
Serum
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Guinea Pig
Immunogen:
Synthetic peptide corresponding to aa450-477 from human cytokeratin 9, coupled to KLH.
Format:
Supplied as a liquid, 0.5% BSA, 0.09% sodium azide.
Extra Details:
CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oe-sophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum. (see references listed below). Applications: Suitable for use in Western Blot and Immunohistochemistry (frozen and paraffin-embedded cells and tissue). Recommended Dilution: Immunohistochemistry: 1:100-1:200; 1:50 for paraffin-embedded tissue; Incubation Time 1 hr at RT; extended with paraffin. Western Blot: 1:5000-1:10,000 (ECL) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice