C9097-42E

Cytokeratin 8 (Cytokeratin-8, CK 8, CK8, CK-8, Cyk8, Cardiac Autoantigen 2 120kD, Card2, Keratin 8, Keratin-8, K8, KRT8, Keratin Type II, Cytoskeletal 8, K2C8, KO)

Size

£659.00

SKU:: C9097-42E
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Cytokeratin 8
Clone:: H1
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
Extra Details:: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen, paraffin): 1:25-1:200 with avidin-biotinylated horseradish peroxidase complex (ABC) as a detecting reagent. Flow Cytometry: 1:25-1;200 Western Blot: 1:100-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice